Dr. Guillaume Beyrend-Frizon

I'm a physician-scientist specializing in tumor immunology and advanced cytometry analysis. My work bridges cutting-edge immunotherapy research with practical tools that make complex data analysis accessible to scientists worldwide.

I created the Cytofast R package for flow and mass cytometry data analysis, which is used by researchers across pharma, biotech, and academic institutions. Learn Cytometry was born from the questions I kept getting from colleagues: "How do I actually analyze my cytometry data in R?"

Publications

• Clinical Rheumatology · 2023 · Spondyloarthritis mass cytometry immuno-monitoring: a proof of concept study in the TiCoSpA trial.
• Cell Reports Medicine · 2023 · OX40 agonism enhances PD-L1 checkpoint blockade by shifting the cytotoxic T cell differentiation spectrum.
• Nature Communications · 2022 · A third vaccination with a single T cell epitope confers protection in a murine model of SARS-CoV-2 infection.
• Frontiers in Immunology · 2022 · Identification of a Disease-Associated Network of Intestinal Immune Cells in Treatment-Naive Inflammatory Bowel Disease.
• Arthritis Research & Therapy · 2021 · Immunoprofiling of early rheumatoid arthritis using mass cytometry reveals an activated basophil subset inversely linked to ACPA status.
• iScience · 2021 · Memory CD8 T cell heterogeneity is primarily driven by pathogen-specific cues and additionally shaped by the tissue environment.
• Frontiers in Immunology · 2020 · Visualizing Dynamic Changes at the Maternal-Fetal Interface Throughout Human Pregnancy by Mass Cytometry.
• Journal for ImmunoTherapy of Cancer · 2020 · Host genetics and tumor environment determine the functional impact of neutrophils in mouse tumor models.
• JoVE · 2019 · Visualization and Quantification of High-Dimensional Cytometry Data using Cytofast, FlowSOM and Cytosplore.
• Journal for ImmunoTherapy of Cancer · 2019 · PD-L1 blockade engages tumor-infiltrating lymphocytes to co-express targetable activating and inhibitory receptors.
• Computational and Structural Biotechnology Journal · 2018 · Cytofast: a workflow for visual and quantitative analysis of flow and mass cytometry data.
• F1000Research · 2018 · Cytomegalovirus infection and progressive differentiation of effector-memory T cells.

About this site

Everything on Learn Cytometry is free. No subscription, no paywall. The 42 chapters and all video tutorials are accessible to everyone — from PhD students running their first CyTOF experiment to experienced immunologists looking to add R-based workflows to their toolkit.