Flow & Mass Cytometry · Free Training

The best two hours
you'll invest in your research.

13 video lessons, 42 written chapters, and all R scripts — by an MD-PhD who built the Cytofast package. Completely free.

✓ Free No subscription. No paywall. Ever.

Watch the Videos Browse the 42 Chapters
13Videos
42Chapters
12Publications
100%Free
Video Tutorials

Two modules, zero prerequisites.

Start from scratch or jump straight to AI-enhanced visualizations — each module is self-contained and includes R scripts.

Module 1 — Beginner R for Cytometry
Module 1
Beginner · Dec 2024
Beginner R for Cytometry

From installing R to clustering analysis and dimensionality reduction. Everything you need to analyze your first FCS file.

📹 10 chapters 📄 R scripts + FCS files
Watch Module 1 →
Module 2 — AI-Enhanced Visualizations
Module 2
Intermediate · Jan 2025
AI-Enhanced Visualizations

Combine Claude AI and R to generate publication-ready graphs — tSNE, heatmaps, dendrograms, and more in minutes.

📹 3 chapters 📄 7 R scripts included
Watch Module 2 →
42 Chapters

A complete cytometry handbook.

From instrument fundamentals to AI-powered single-cell analysis — structured as a complete learning path.

Part I
Foundations
Chapters 1–10
  • Introduction to mass cytometry
  • High-dimensional data
  • CyTOF instrumentation
  • Panel design & sample prep
  • Quality control & IMC
Read chapters →
Part II
Data Analysis
Chapters 11–15
  • Dimensionality reduction
  • UMAP & tSNE
  • FlowSOM & PhenoGraph
  • Trajectory analysis
  • Machine learning
Read chapters →
Part III
Clinical Applications
Chapters 16–30
  • Tumor microenvironment
  • Autoimmune diseases
  • Vaccine evaluation
  • Transplantation immunology
  • Infectious diseases
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Part IV
Advanced Topics
Chapters 31–42
  • Multi-omics integration
  • AI & mass cytometry
  • Spatial proteomics
  • Epigenetic profiling
  • Claude AI + R workflows
Read chapters →
Browse all 42 chapters →
Dr. Guillaume Beyrend-Frizon
Dr. Guillaume Beyrend-Frizon
MD-PhD in Tumor Immunology · Leiden University Medical Center
Creator of Cytofast R package Editor at Frontiers in Immunology Tumor immunology
12Publications
500+Scientists

Need expert cytometry consulting?

MD-PhD expertise in tumor immunology and advanced single-cell analysis. Supporting pharma, biotech, and academic teams worldwide.

Tumor Immunology Single-Cell Analysis Panel Design Data Analysis AI Integration
Get in touch →

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Because modern medicine deserves tools at its level

🔓 Free 🩺 Comprendre-Santé

Vulgarisation médicale de qualité — histoire de la médecine, grandes découvertes, articles en français.

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AI assistant for emergency medicine — standardized scripts, clinical decision support for emergency physicians.

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