Your First Video Training as VIP member
💫 Transform Your Cytometry Data Analysis Skills with R
Welcome to the most comprehensive online course for analyzing flow and mass cytometry data using R. This isn’t just another programming course – it’s your gateway to mastering immunological data analysis with confidence and precision.
❓Why This Course Is Different
Unlike generic R tutorials, we focus exclusively on cytometry analysis, making complex immunological data interpretation accessible and practical. Our course is designed by immunologists for immunologists, bridging the gap between raw data and meaningful insights.
We know exactly where you might stumble and how to help you succeed. This unique guidance system saves you countless hours of troubleshooting and frustration.
🌱 Don't Just Learn R – Master Cytometry Analysis
Regular users can access basic features, but VIP members unlock the full potential of their cytometry data analysis. Join the community of successful researchers who have transformed their data analysis capabilities through our comprehensive training.
Ready to Elevate Your Research?
Secure your VIP access now and start your journey to becoming a cytometry analysis expert. Remember, your success in data analysis is our priority – we’re here to support you every step of the way.
Contact us for institutional licenses and group discounts.
🎓 What You'll Master:
• Unbiased analysis of flow, mass, and spectral flow cytometry data
• Advanced clustering techniques for pattern recognition
• Custom visualization methods for publication-ready figures
• Automated workflows for high-throughput analysis
• Real-world applications using published datasets
Clustering techniques: FlowSOM, Phenograph
Dimensionality reduction: tSNE, UMAP
Cell trajectory: Diffusion Map

📝 The Learning Experience:
Our step-by-step approach means zero prior R experience is needed.
We start from the basics and progressively build your expertise through:
– Crystal-clear video tutorials
– Hands-on practice with real datasets
– Comprehensive written guides
– Interactive problem-solving sessions
🎯 Real Research Impact:
Break Free from Software Limitations:
Unlock the power to create publication-worthy analyses exactly how you envision them. While commercial software keeps you in a box, our R-based approach empowers you to:
- Design stunning custom visualizations that tell your data’s unique story
- Develop innovative analysis pipelines that set your research apart
- Create reproducible workflows that journals increasingly demand
- Build beautiful figures that perfectly match your publication’s needs
Learn with Real-World Data:
Why practice with simplified examples when you can master complex, published datasets? We provide you with an extensive collection of 24 real-world files (16 flow cytometry and 8 mass cytometry datasets) straight from peer-reviewed research. These aren’t just training files – they’re the same datasets that have already proven their worth in high-impact publications. This means you’ll:
- Work with genuine biological complexity, not oversimplified examples
- Navigate the same challenges you’ll face with your own research data
- Understand how to handle technical variations and experimental nuances
- Develop confidence in analyzing data that meets publication standards
Getting the Most Out of Your Learning Experience
Learn effortlessly with our comprehensive course materials designed to maximize your learning experience. Each video lecture provides real-time demonstrations in R, showing you exactly how to implement every technique step by step. No need to worry about transcribing code – every video comes with a complete R script containing all the code you see demonstrated. This means you can focus on understanding the concepts rather than typing.
To ensure hands-on practice, we provide all the necessary materials for you to follow along:
- Detailed video demonstrations
- Ready-to-use R scripts matching the video content
- Sample FCS files for practice
- Corresponding metadata files for complete analysis
This practical approach allows you to reproduce everything you see in the videos on your own R software. You can pause, practice, and perfect each technique at your own pace. Whether you prefer to type the code yourself for deeper learning or use the provided scripts to focus on understanding the concepts, you have the flexibility to learn in the way that works best for you.
Our goal is to make your learning process smooth and practical, giving you everything you need to successfully apply these techniques to your own research data once you complete the course.
Chapter 1:
Unlock the full potential of your cytometry data with this specialized course designed to take you from basic analysis to advanced visualization techniques. See exactly what you’ll achieve, from elegant heatmaps to sophisticated clustering analyses that will set your research apart.
Chapter 2:
Transform your computer into a powerful cytometry analysis workstation with our foolproof setup guide. No more installation headaches – just clear, step-by-step instructions to get you analyzing data in record time.
Chapter 3:
🚀 Step into the exciting world of AI-powered cytometry analysis as we combine the intelligence of Claude with the visualization power of R to create your first custom graphs! 🎨 This hands-on chapter will guide you through every step of the process, showing you how this magical combination can transform your data into beautiful, meaningful visualizations – a skill that will revolutionize how you analyze and present your flow cytometry results. ✨
Chapter 4:
Take command of your FCS files like never before, with the power to modify and optimize every aspect of your data. Learn the fundamental skills that will set you free from the constraints of commercial software.
Chapter 5:
Transform your raw flow cytometry data into crystal-clear visualizations using the most advanced pre-processing techniques available. Learn methods that have been validated by the scientific community and are perfect for your unique data.
Chapter 6:
Navigate the complexity of mass cytometry data with ease, mastering techniques specifically designed for handling dozens of markers simultaneously. Transform complicated datasets into clear, actionable insights.
Chapter 7:
Step into the future of cytometry analysis with cutting-edge clustering techniques like Phenograph and flowSOM. Discover new cell populations and patterns that traditional analysis methods might miss.
Chapter 8:
Create stunning visualizations that instantly communicate your findings through professional heatmaps and intuitive graphs. Master the art of turning complex data into clear, compelling visual stories.
Chapter 9:
Turn the complexity of your data into an intuitive visual journey using state-of-the-art dimensionality reduction approaches. From cell development pathways to population dynamics, you’ll reveal the hidden narratives within your experiments.
Chapter 10:
Complete your journey to cytometry analysis mastery with this comprehensive application of all techniques to mass cytometry data. Emerge ready to tackle any cytometry analysis challenge with confidence and expertise.