The integration of epigenetic analysis with mass cytometry has opened new frontiers in understanding cellular heterogeneity and gene regulation at the single-cell level. This chapter explores the exciting developments in combining these powerful technologies, with a focus on histone modification profiling, DNA methylation analysis, and applications in tumor immunology.
Combining Mass Cytometry with Epigenetic Analysis
The landmark study by Cheung et al. (2018) published in Cell demonstrated the power of combining mass cytometry with epigenetic profiling : “Single-Cell Chromatin Modification Profiling Reveals Increased Epigenetic Variations with Aging”. They developed a technique called EpiTOF (Epigenetic landscape profiling using cytometry by time-of-flight) to analyze 40 chromatin markers in single cells. This study revealed increased epigenetic variation in immune cells with aging, providing unprecedented insights into the epigenetic basis of immune system changes over the lifespan.
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