The advent of spatial transcriptomics and proteomics has ushered in a new era of biological understanding, where the location of cells within tissues is as important as their molecular profiles. This chapter explores the exciting intersection of imaging mass cytometry with spatial transcriptomics, offering unprecedented insights into tissue architecture and cellular interactions.
Single-Cell Spatial Analysis in Situ
The ability to perform single-cell analysis within the native tissue environment has opened new avenues for understanding cellular behavior and interactions.
A landmark study by Keren et al. (2018) in Cell, cited over 830 times, “A Structured Tumor-Immune Microenvironment in Triple Negative Breast Cancer Revealed by Multiplexed Ion Beam Imaging,” used highly multiplexed ion beam imaging to analyze breast cancer samples at single-cell resolution. This work revealed complex spatial relationships between tumor and immune cells, providing insights into tumor progression and potential therapeutic targets. It is actually another version of the CyTOF, but where spatial resolution has been permitted.
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